The Maribyrnong catchment is located to the north-west of Melbourne. Water flows from the headwaters around Mount Macedon to form Jacksons Creek, which flows into Rosslynne Reservoir near Gisborne. Water is released from Rosslynne Reservoir back into Jacksons Creek, and where it joins with Deep Creek (flowing from the north) the Maribyrnong River is formed at Keilor North. The river runs south through Yarraville in inner Melbourne before meeting the Yarra River and flowing into Port Phillip Bay.
The Maribyrnong system continues to be an important place for Traditional Owners and their Nations. The Registered Aboriginal Party (RAP) in the Maribyrnong River area is the Wurundjeri Land and Compensation Cultural Heritage Council Aboriginal Corporation. RAP representatives were involved in preparing the Maribyrnong system seasonal watering proposal.